Chemical Laboratory Instrumentation: Operation, Quality and Safety of Equipment



Details :

Modern chemical laboratories rely on advanced instrumentation, strict quality systems, and rigorous safety practices to ensure accurate and reliable results. This course provides a comprehensive understanding of laboratory analytical techniques, instrumentation operation, troubleshooting, quality assurance based on ISO standards, and safe handling of chemicals and equipment.

Participants will gain both theoretical knowledge and practical insights into operating laboratory instruments such as chromatography systems, spectroscopy tools, and analytical equipment, while maintaining compliance with international quality and safety standards.

 

Ø  By the end of this course, participants will be able to:

  • Understand core chemical laboratory techniques and analytical methods
  • Operate and interpret results from chromatographic and spectroscopic instruments
  • Apply quantitative analysis and calibration techniques
  • Troubleshoot common laboratory instrumentation issues
  • Implement ISO 17025 laboratory quality requirements
  • Ensure compliance with laboratory safety and OSHA standards
  • Manage chemical hazards, waste, and emergency situations effectively 


  • Laboratory Analysts & Technicians
  • Chemists and Quality Control Personnel
  • Process and Production Engineers
  • HSE Officers working in laboratories
  • Research & Development Staff 

Day 1: Fundamentals of Chemical Laboratory Techniques & Chromatography

Session 1 

  • Introduction to chemical laboratory techniques
  • Methods of analysis (qualitative vs quantitative)
  • Basic laboratory techniques:
    • Sample preparation
    • Analytical measurement
    • Fundamental concepts & chemical equations
    • Acidity, pH, and buffer systems

Session 2 

  • Preparation of chemicals and reagents
  • Titration methods (acid-base, redox, complexometric)
  • Manipulation techniques:
    • Solid Phase Extraction (SPE)
    • Derivatization
  • Introduction to chromatography:
    • Principles and types
  • Gas Chromatography (GC):
    • Injection systems
    • Columns
    • Detector types

Day 2: Advanced Instrumentation & Spectroscopy

Session 1 

  • High Performance Liquid Chromatography (HPLC):
    • Mobile phase selection
    • Pumping systems
    • Sample injection systems
    • Columns and detectors
  • Data management software in laboratories
  • Analytical retention processes

Session 2 

  • Spectroscopy techniques and molecular identification:
    • UV/Vis spectroscopy
    • Infrared (IR) and Raman spectroscopy
    • Mass spectrometry
    • X-ray and electron spectroscopy
    • Flow injection analysis
  • Ion chromatography and pH probes
  • Atomic Absorption Spectroscopy (AAS)
  • Nuclear Magnetic Resonance (NMR):
    • Design and operating principles
    • Applications
    • Multi-dimensional NMR
    • Solid-state NMR

Day 3: Troubleshooting, Quantitative Analysis & Quality Systems

Session 1 

  • Instrument troubleshooting:
    • Column contamination
    • Peak broadening
    • Ghost peaks and system peaks
    • Inlet filter contamination
    • Mobile phase issues (HPLC)
    • AAS interferences
    • Detector contamination
  • Quantitative analysis methods:
    • Calibration techniques
    • External vs internal standards
    • Standard addition method

Session 2 

  • Statistical analysis in laboratory work:
    • Outlier tests
    • Error analysis
    • Confidence limits
    • Detection limits
    • Repeatability and reproducibility
  • Laboratory Quality Systems:
    • ISO 17025 requirements (management & technical)
    • Validation of analytical methods
    • Laboratory accreditation
  • Introduction to laboratory safety:
    • Hazard identification
    • Safe work practices

Day 4: Laboratory Safety & Practical Application

Session 1 

  • Management of compressed gases
  • Safe transport of chemicals
  • Electrical safety in laboratories

Session 2 

  • Practical Session / Site Visit
    • Instrument observation (HPLC, GC, AAS, etc.)
    • Safety systems review
    • Hands-on discussion on operational challenges

Day 5: Laboratory Safety Standards & Emergency Response

Session 1 

  • Guidelines for Good Laboratory Practices (GLP)
  • OSHA laboratory standards
  • Laboratory fire protection systems
  • Storage and handling of:
    • Flammable liquids
    • Combustible chemicals

Session 2 

  • Laboratory waste management:
    • Hazardous waste disposal
    • Chemical waste segregation
  • Spill management procedures
  • Emergency response and laboratory first aid 
  • Interactive lectures
  • Case studies from real laboratory scenarios
  • Group discussions
  • Instrument demonstrations (if available)
  • Practical session / site visit
  • Problem-solving and troubleshooting exercises 

daily 09:00 – 14:00